In the present research work novel series of heterocyclic like 2, 5, 6 substituted triazolo-thiadiazole with metal-ligands complexes (Zinc and Copper) were synthesized. The heterocyclic incorporated with isoniazide which then evaluated for antimicrobial. The method of synthesis of some triazolo-thiadiazoles involves the reaction between Thiosemicarbazide (I) and substituted aromatic acids undergo cyclization to give 2-amino-5-aryl-1, 3, 4-thiadiazole (II). Again its Schiff base 5-phenyl-N-[(1Z)-phenylmethylene]-1,3,4-thiadiazol-2-amine (III) reaction is done with aromatic aldehydes by alkyl-iminodeoxobisubstitution. Then 2, 5, 6 substituted Triazolo-thiadiazole (IV) derivatives are prepared by using and Isoniazide Anhydrous FeCl3. Various derivatives were prepared with different aromatic acids (salicylic acid, Nicotinic acid and Para amino benzoic acid) and aromatic aldehydes (4-OH-Benzaldehyde and Furfural). And then in the last step the metal complexes (V) were prepared with CuCl2 and ZnCl2 reflux in ethanol with drop wise addition of alcoholic ammonia to raise the pH of mixture to 6.5. The synthesized compounds were characterized by IR, Mass, 1H-NMR, UV-visible spectrum and Elemental Analysis. After synthesis the chemical compounds were screened for their biological activity against gram Negative and gram positive bacteria and also fungi using agar-well diffusion method. Compounds (1A1, 1B1, 2B1, 3B1) were found to be the most effective compound against fungal microorganisms. Compounds (1A2, 1B2, 2A2, 3B2) were found to be more effective against bacterial microorganisms. All the compounds were found to be nearly Shown promising antimicrobial activities.
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